Personal tools
/ Departmental Items / Physics Colloquium / High-Speed Atomic Force Microscopy: The Dawn of Dynamic Structural Biochemistry

High-Speed Atomic Force Microscopy: The Dawn of Dynamic Structural Biochemistry

— filed under:
Date and time Mar 15, 2018
from 04:10 PM to 05:00 PM
Location 60 St. George Street, MP 102
Host Claudiu Gradinaru

Simon Scheuring

Weill Cornell Medicine

ScheuringThe advent of high-speed atomic force microscopy (HS-AFM) has opened a novel research field for the dynamic analysis of single unlabeled bio-molecules. The integration of a non-disturbing buffer exchange system, allowing the gradual change of the environmental conditions during HS-AFM operation, provides a breakthrough towards the performance of structural titration experiments. Analyzing membrane proteins in membranes and under controlled exposure to environmental stimuli, we report conformational changes of a transporter in response to substrates, of receptors and of ion channels upon ligand binding. Altogether, we showcase the unique potential of HS-AFM, providing ~1nm lateral, ~0.1nm vertical and ~100ms temporal resolution, for visualizing the dynamics of membrane proteins, label-free and in close-to-native conditions.

 

Document Actions