Distinguished Visiting Scientist lecture of the Institute for Optical Sciences

Distinguished Visiting Scientist lecture of the Institute for Optical Sciences

The interaction of G-protein-coupled receptors with G proteins is a key event in transmembrane signal transduction that leads to vital decision-making by the cell.

The utilization of single-molecule techniques has become widespread in biophysics labs. In this introductory lecture we will review the physical basis of the methodology and learn about the various applications in the biosciences, as well as it’s limitations given by the optical and photophysical constraints in fluorescence imaging. Further, we will discuss in which way such imaging data yield information about clustering and molecular motility and how one can extract those.

With the development of photoswitchable labels in combination with single-molecule imaging it recently became possible to perform optical microscopy on the 10 nm scale, far below the classical diffraction limit.

Distinguished Visiting Scientists lecture of the Institute for Optical Sciences

Distinguished Visiting Scientists program of the Institute for Optical Sciences

Distinguished Visiting Scientists lecture of the Institute for Optical Sciences

Distinguished Visiting Scientists Lecture of the Institute for Optical Sciences

Distinguished Visiting Scientists Lecture of the Institute for Optical Sciences

2011 Boris P. Stoicheff Lecture and Distinguished Visiting Scientists Lecture of the Institute for Optical Sciences