Recent developments in microscopy have enabled powerful insights into local molecular, cellular, and tissue-level structure and dynamics. Our lab has been focusing on the development and application of several combinatorial microscopy platforms, including super- resolution, light sheet/selective plane illumination, and full-field optical coherence tomography. The application of these tools to studies of biomolecular self-assembly , membrane receptor self-association, protein-protein interactions, and developmental processes will be described. A number of the key technical challenges that underpin these approaches will similarly be discussed.